Anti-Leishmania major activity of Calotropis procera extract by increasing ROS production and upregulating TNF-α, IFN-γ and iNOS mRNA expression under in vitro conditions

Table 1 The specific primers for β-actin, cytokine (IFN-γ and TNF-α) and iNOS genes

Primer name	Sequence (5' to 3')	Base/length	TA (˚C)	Product size (bp)
β-Actin—forward	TGCCGACAGGATGCAGAAG	19	58	106
β-Actin—reverse	GCCGATCCACACGGAGTACT	20
IFN-γ- forward	AGCTCTGCATCGTTTTGGGTT	21	54	118
IFN-γ- reverse	GTTCCATTATCCGCTACATCTGAA	24
TNF-α-forward	CACGCTCTTCTGCCTGCTG	19	56	105
TNF-α-reverse	GATGATCTGACTGCCTGGGC	20
iNOS- forward	ATAGAGGATGAGCTGAGCATTCCA	24	58	109
iNOS- reverse	GCGTTACTCCACCAACAATGGCAA	24

ISSN: 1349-4147