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Fig. 3 | Tropical Medicine and Health

Fig. 3

From: Anti-Leishmania major activity of Calotropis procera extract by increasing ROS production and upregulating TNF-α, IFN-γ and iNOS mRNA expression under in vitro conditions

Fig. 3

ROS production in C. procera leaf extract- treated L. major. Promastigotes were treated with 100 ( < IC50), 222.44 (IC50-72 h) and 377.28 (IC50-24 h) µg/mL for 24, 48 and 72 h at 25 ± 1  C. Cells (control and treated cultures) were incubated with probe H2DCFDA (green fluorescent dye). Intracellular ROS production was analyzed by flow cytometry. Data are expressed as mean of DCF fluorescence intensity ± standard deviation. Significant statistical differences in relation to control are indicated as (*), (**), and (***) at the 0.05, 0.01 and 0.001 levels, respectively

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Tropical Medicine and Health

ISSN: 1349-4147

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