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Malaria research in the Central African Republic from 1987 to 2020: an overview



The national malaria control policy in the Central African Republic (CAR) promotes basic, clinical, and operational research on malaria in collaboration with national and international research institutions. Preparatory work for the elaboration of National Strategic Plans for the implementation of the national malaria control policy includes developing the research component, thus requiring an overview of national malaria research. Here, this survey aims to provide an inventory of malaria research as a baseline for guiding researchers and health authorities in choosing the future avenues of research.


Data sources and search strategy were defined to query the online Medline/PubMed database using the “medical subject headings” tool. Eligibility and study inclusion criteria were applied to the selected articles, which were classified based on year, research institute affiliations, and research topic.


A total of 118 articles were retrieved and 51 articles were ultimately chosen for the bibliometric analysis. The number of publications on malaria has increased over time from 1987 to 2020. These articles were published in 32 different journals, the most represented being the Malaria Journal (13.73%) and the American Journal of Tropical Medicine and Hygiene (11.76%). The leading research topics were drug evaluation (52.94%), expatriate patients (23.54%), malaria in children (17.65%), morbidity (13.7%), and malaria during pregnancy (11.76%). The publications’ authors were mainly affiliated with the Institut Pasteur of Bangui (41%), the French Military Medical Service (15.5%), and the University of Bangui (11.7%). Collaborations were mostly established with France, the UK, and the USA; some collaborations involved Switzerland, Austria, Pakistan, Japan, Sri Lanka, Benin, Cameroun, Ivory Coast, and Madagascar. The main sources of research funding were French agencies (28.6%) and international agencies (18.3%). Most studies included were not representative of the whole country. The CAR has the capacity to carry out research on malaria and to ensure the necessary collaborations.


Malaria research activities in the CAR seem to reflect the priorities of national policy. One remaining challenge is to develop a more representative approach to better characterize malaria cases across the country. Finally, future research and control measures need to integrate the effect of COVID-19.


Malaria remains a major public health problem; this disease is widespread in tropical regions despite increased funding for control programs and major advancements in diagnosis and treatment. Although the prevalence of malaria has decreased recently in parts of Africa, it continues to increase in the Central African Republic (CAR): prevalence increased from 68.9% to 74.71% between 2015 and 2020 [1]. Over the last 20 years, many initiatives have been launched in the CAR, particularly between 2000 and 2013—a period of intensification of malaria control actions—when the CAR benefited from some World Health Organization (WHO) programs and those of other international non-governmental organizations. Initiatives taken in the CAR include the introduction of artemisinin-based combination therapies as the first-line treatment for unconfirmed malaria in 2005, the establishment of a network of community health workers (CHWs) in 2008, the introduction of a rapid diagnostic test (RDT) in 2009, the distribution of long-lasting insecticide-treated nets (LLINs) in 2010, and home-based management of malaria (PECADOM) starting in 2013 [2, 3].

The WHO’s Global Technical Strategy for malaria (GTS) 2016–2030 produced the High Burden to High Impact country-led approach whose actions are tailored to local data and information [4]. Thus, in 2016, the CAR set up a national malaria control policy derived from the GTS; this policy emphasizes policy support through catalyzing innovation and developing malaria research. In addition, this national policy strives to promote basic, clinical, and operational research on malaria at all levels of the health system in collaboration with national and international research institutions. This policy will be implemented through National Strategic Plans (NSP) [5]. The preparatory work for the elaboration of the NSP for malaria surveillance and the development of malaria scientific research in CAR requires an overview of national malaria research.

The challenges for malaria research are numerous in the CAR. The priority and the most pressing issues involve vector control. For example, a study in Bangui showed that mosquitoes can modify their behavior to blood-feed at places and times when humans are not protected, i.e., primarily outside the home environment [6]. In addition, there is a risk that Anopheles stephensi—already present in Sudan—becomes established in the CAR; this vector frequents urban areas. However, knowledge on malaria vectors remains limited in the CAR, thereby hampering disease control [7]. Another important issue is insecticide or drug resistance. A recent study showed multiple insecticides resistance in important malaria vectors (An. gambiae s.l. and An. funestus s.l.) in Bangui [8, 9]. Likewise, there is a multiplicity of factors that may favor the introduction, the emergence and the selection of drug-resistant malaria strains, particularly those resistant to artemisinin derivatives. These factors include the presence of troops from Cambodia, Thailand, Bangladesh, Bhutan and Nepal for the past 10 years in the CAR; all of these countries are located in the area where artemisinin resistance has emerged or neighbor this epicenter of emergence, thus leading to the potential spread of multidrug-resistant P. falciparum. Finally, the reservoir of asymptomatic carriage has never been assessed and is therefore an important issue to address to evaluate the potential of residual malaria transmission.

Here, we provide an inventory of malaria research as a baseline to help researchers and health authorities define the avenues for future malaria research in the CAR. This study establishes a database defining the current state of art with a list of the main articles on malaria in a country that has very limited internet connections and all but lacks medical research documentation centers or up-to-date libraries. Research results are commonly communicated in the form of articles published in journals indexed in international bibliographic databases of which the most recognized in biomedical sciences is “Medline” with its electronic interface “PubMed” [10]. This literature review provides a bibliometric profile of the publications in Medline/PubMed on malaria in the CAR over a period of 33 years (from 1987 to 2020).


Data source and search strategy

An online Medline/PubMed database search was conducted from 1 November to 31 January 2021 (last update). The Medline/PubMed database is based on the US National Library of Medicine® (NLM) bibliographic database, which holds more than 28 million references to journal articles in life sciences, published from 1966 by more than 5,200 journals worldwide [11]. The medical subject headings (MESH) tool was used as follows: (((malaria) OR plasmodium) OR anopheles) AND ("Central African Republic" OR "Bangui"))). The retrieved references were then analyzed for the following keywords: malaria, mosquito, antimalarial drug, clinical malaria.

Eligibility, study inclusion criteria and analysis plan

Retrieved publications were screened to select the reports of primary original studies conducted between 1966 and 2020 exclusively in the CAR. English and French language publications were included. These publications had to refer specifically to human malaria. Articles involving other regions, other pathologies, or animal malaria were excluded. Two authors (R.N-B and G.V) assessed all studies and agreed on those that met the criteria and should therefore be included. Data collected included the number of articles published annually, journal, author affiliations, funding, city of study, types of research (basic, clinical, epidemiological research and health policy and systems research) and research topics. A data extraction form was designed to compile the information available in the publications. Two authors (R.N-B and G.V) independently extracted data from the studies using this form. Any discrepancies in data extraction by the two authors were reconciled through discussion. The data obtained were encoded and processed in Microsoft Excel 2010.


Overview of selected articles

A total of 118 articles were identified and recorded in the Medline/PubMed database. We discarded 21 articles investigating other pathologies, 34 articles about malaria in the global subregion of central Africa—with or without data from the CAR—10 articles on animal malaria, 1 article with no full text available and 1 article that was a duplicate study. Ultimately, 51 articles were included in our assessment of research on malaria in the CAR (Fig. 1). Most of the retrieved articles were written in English (39/51).

Fig. 1
figure 1

Flowchart of study selection process and search strategy

Trends in malaria research and journals

The first report on malaria in the CAR being published in 1987, Fig. 2 shows the increase in the number of articles published each year. Malaria research in the CAR can be grouped into three phases: 2005–2006 with 3–4 papers per year; 2015–2017 with 4–5 papers per year and 2017 with 4 papers per year. Thus, in 33 years of malaria research in the CAR, there were on average 1.55 articles per year. Over the last 5 years, there has been an increase, with an average of 3.6 articles per year.

Fig. 2
figure 2

Trend of malaria publications in Central African Republic of 1987 at 2020

These articles were published in 32 different journals, the leading journals being the following: Malaria Journal (13.73%), American Journal of Tropical Medicine and Hygiene (11.76%), Bulletin de la Societe de Pathologie Exotique (7.84%), Médecine Tropicale (5.88%) and Transactions of the Royal Society of Tropical Medicine and Hygiene (5.88%) (Table 1).

Table 1 Journals publishing malaria research from Central African Republic

Research topics addressed in the selected articles

Most publications involved clinical research (41.7%). Epidemiological and basic research accounted for 23.52% and 21.56% of the articles, respectively. Health policy and systems research was less represented (13.22%).

Regarding the research topics, drug evaluation (efficacy and resistance) was the most common topic of investigation with 27 publications (52.94%) (Fig. 3). Publications on expatriate patients (23.54%), children (17.65%), and morbidity (17.65%) were followed by papers on prevention (13.72%) and malaria during pregnancy (11.76%). Less frequently addressed topics included severe malaria cases (7.84%), vector control (7.84%), diagnosis (3.9%), Plasmodium genotype (3.9%), and co-infection (3.9%) (Table 2). There were no studies on immunology or vaccine development in the selected articles.

Fig. 3
figure 3

Areas of focus for malaria research in Central African Republic

Table 2 Summary of the studies included in the review

Affiliations and funding

First authors were affiliated with CAR institutions and French institutions in 32 (62.74%) and 9 (17.64%) publications, respectively. The other authoring institutions were located in the UK, the USA, Switzerland, Pakistan, Japan, Sri Lanka, and Ivory Coast. Last authors were affiliated with CAR and French institutions in 17 (38.6%) publications each, and the other last authors’ institutions were located in the USA, Switzerland, Cameroun, Japan, Sri Lanka, and Madagascar. The reported studies were mainly carried out at the Institut Pasteur of Bangui (41%), the University of Bangui (11.7%), French Military Medical Services (15.5%), and the CAR Ministry of Public Health (5.8%). Other co-authoring institutions were Aix-Marseille University, Bangui National Hospital, Bouake Regional Hospital, Georgetown University Hospital, Center of Disease Control and Prevention in Atlanta (USA), and the University of Colombo. Twenty-seven (53%) of these studies were carried out in collaboration between several institutes and research centers; among them, 12 involved more than two institutions (28.6%). Funding was acknowledged in only 28 publications (55%), of which 9 benefited from cofunding (21.4%). The main sources of these funds were French agencies (28.6%) and international agencies (18.3%).


The bibliometric analysis focused on a review of studies to assess the type and amount of malaria research conducted in CAR from 1987 to 2020. The publication of the first paper on malaria in the CAR coincided with the spread of chloroquine resistance in Africa in the 1980s, and the three articles of this period addressed chloroquine resistance. Similarly, in other areas of central Africa (i.e., the Democratic Republic of Congo, the Republic of Congo, and Cameroon), numerous publications on chloroquine resistance have been published [6163]. The annual number of CAR malaria articles published varied from year to year and has been increasing since 1987. The introduction of artemisinin in the treatment regimen beginning in the 2000s led to assessments of its efficacy and of other antimalarial drugs, explaining the number of articles published between 2005 and 2006 involving the assessment of antimalarial drugs. From 2015 to 2017, there was an average of four articles per year, corresponding to the publication of the first articles on the malaria vectors and the concurrent deployment of soldiers for peacekeeping missions in the CAR during the periods of unrest in 2013, and ending with the publications on imported malaria cases in 2017. This study showed that the top two journals, Malaria Journal and the American Journal of Tropical Medicine and Hygiene were the same as those reported in a bibliometric analysis of malaria research in Malawi from 1984 to 2016 [64]. A bibliometric analysis of the literature on malaria vector resistance from 1996 to 2015 showed that Malaria Journal was the leading journal, although it was established only in 2002 [65].

Almost all the studies reported in the articles were derived from primary research, accounting for 50 out of 51 papers (98%). The types of malaria research conducted in the CAR were exactly the same types of malaria research carried out in Malawi, with mostly clinical research and few health policy and systems research studies [64]. Clinical research has been revealed in bibliometric analysis of research on meningitis and other infectious diseases as the leading type of approach and topic of research [66]. The research topics and areas of focus for malaria research in the CAR matched the priorities of the topics listed in the national policy and the ground situation in the country, namely drug evaluation, and malaria in children and in pregnant women. However, efforts must be made in the field of vector control and diagnosis, which are the pillars of any control strategy, in particular regarding parasite genotype, immunological profile, and vaccine development. Drug evaluation was also the leading research topic in a study carried out in the Greater Mekong subregion [67].

Another important aspect revealed in this bibliometric analysis was the level of collaboration. Based on a review of publications, our survey revealed a high rate of collaboration between a CAR institution and one or more foreign/international institutions. Research institutes on almost every continent have contributed to the study of malaria in the CAR and this multiple and diversified collaboration strengthens the local capacity for malaria research and documents the local malaria situation. International funding was acknowledged in more than half of the articles selected and no local funding was cited. In contrast, in a study on the investment in malaria research in sub-Saharan Africa, Tanzania, Uganda, Kenya, Malawi, and Ghana are all countries showing greater funding, with the CAR being ranked among countries with no funds allocated for research [68]. This lack of investment may also explain the low scientific production regarding malaria research in the CAR compared with other endemic countries. Furthermore, the limited resources and expertise in the CAR means that the country depends on international collaboration to ultimately control the fatal infectious disease of malaria.

We found that 70% of the studies on malaria in the CAR had been carried out in the capital (Bangui), probably because the city is readily accessible to foreign researchers. However, the representativeness of these studies may be called into question, because Bangui has about 812,407 inhabitants for an area of 67 km2, whereas the vast majority of the CAR’s 6,091,097 inhabitants are spread across an area of 623,000 km2 [69]. Restricting studies to Bangui can be attributed to the lack of qualified infrastructures in the outlying provinces and especially to conflicts, unrest, and the military crisis, which have a greater impact in the provinces. Notwithstanding, a study carried out in the network of sentinel sites set up for epidemiological surveillance in the CAR revealed spatial variability in malaria prevalence, with high prevalence in rural and semi-urban areas [14]. Nevertheless, this bibliographic survey provides a sound scientific basis for guiding future control measures against malaria in the CAR and allowed domestic experts to identify the needs for research on malaria in this geographically and digitally landlocked country, with no direct access to broadband internet and connectivity issues.

Finally, future research and control measures will require integrating the effect of COVID-19. In terms of control measures, the COVID-19 pandemic has severely affected health systems in general, including the malaria program with decreased access to LLINs and antimalarial drugs, two key malaria control measures, leading to an increase in morbidity (estimated at 5% to < 10%) and an increase in mortality (estimated at 40% to < 60%) in the CAR, in 2020 compared to the pre-COVID-19 period [70]. Furthermore, the COVID-19 pandemic has also affected the progress of malaria research in the CAR in various ways. Clinical malaria research most carried out in the CAR has been discontinued because individuals have stopped attending health facilities out of fear of exposure to COVID-19, or CHWs and other healthcare staff avoid working in close proximity to febrile patients who may have a high risk of COVID-19 infection. The extensive and uncontrolled use of chloroquine, hydroxychloroquine, and artemisinin derivatives—all antimalarial drugs—and azithromycin—an antibiotic with an antimalarial effect—during the COVID-19 pandemic in the CAR may affect the malaria treatment regimen An interventional study with a combination of control strategies involving vector control, vector surveillance and parasite drug resistance monitoring is to be considered primarily for a host–pathogen–vector–environment interaction approach.


In summary, the increase in the number of malaria-related articles in the CAR is promising, because various aspects of malaria research have been studied. The CAR has a capacity for malaria research and can rely on fruitful collaborations. Unfortunately, most studies had been carried out on the population in Bangui, revealing a lack of representativeness of these studies for the rest of the country. Malaria research activity in the CAR may reflect the priorities of the national policy, but efforts must be made to focus on vector control and diagnosis. This survey provides a sound scientific basis for domestic public health experts to refine and optimize their bibliographic research on malaria. The challenge now lies in developing the representativeness of studies, assessing the immunological profile of the population and the burden of asymptomatic malaria, as well as characterizing the residual reservoir and measuring transmission rates, including anthropological considerations, and developing educational support actions. Future research and control measures need to integrate the effect of COVID-19.

Availability of data and materials

The database of this study is available from the corresponding author upon request.



World Health Organization


Community Health Workers


Rapid diagnostic test


Long-lasting insecticide-treated nets


Home-based management of malaria


Global Technical Strategy for malaria


National Strategic Plans


National Library of Medicine


Medical subject headings


Coronavirus disease 2019


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RNB and JBLD conceived and designed the study. GV, RKIL and AM contributed to the design and analysis plan. RNB and GV collected and analyzed data, drafted the manuscript. All authors interpreted, critically the data. All authors and read and approved the final manuscript.

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Nzoumbou-Boko, R., Velut, G., Imboumy-Limoukou, RK. et al. Malaria research in the Central African Republic from 1987 to 2020: an overview. Trop Med Health 50, 70 (2022).

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