Table 4 Purification profile of E. ocellatus metalloproteinase, serine protease and phospholipase A2 fractions
From: Toxicological analyses of the venoms of Nigerian vipers Echis ocellatus and Bitis arietans
Venom fraction | Purification step | Total protein (µg) | Total activity (µmol/min) | Specific activity (µmol/min/mg) | Purification fold | Yield (%) |
|---|---|---|---|---|---|---|
SVMP | ||||||
| Â | Crude venom | 2.36 | 6.40 | 2.71 | 1.00 | 100.00 |
| Â | Acetone precipitation | 0.92 | 2.64 | 2.87 | 1.06 | 41.30 |
| Â | Ion exchange chromatography on DEAE cellulose | 0.54 | 1.99 | 3.69 | 1.36 | 31.10 |
| Â | Gel filtration on Sephadex G-75 | 0.32 | 1.64 | 5.13 | 1.89 | 25.63 |
SVSP | ||||||
| Â | Crude venom | 2.36 | 5.42 | 2.22 | 1.00 | 100.00 |
| Â | Acetone precipitation | 0.95 | 2.64 | 2.77 | 1.24 | 48.70 |
| Â | Ion exchange chromatography on DEAE cellulose | 0.50 | 1.50 | 3.00 | 1.35 | 27.70 |
| Â | Gel filtration on Sephadex G-75 | 0.39 | 1.27 | 3.26 | 1.46 | 23.43 |
PLA2 | ||||||
| Â | Crude venom | 2.36 | 4.73 | 2.00 | 1.00 | 100.00 |
| Â | Acetone precipitation | 1.82 | 3.94 | 2.16 | 1.10 | 83.30 |
| Â | Ion exchange chromatography on DEAE cellulose | 1.43 | 3.62 | 2.53 | 1.27 | 76.56 |
| Â | Gel filtration on Sephadex G-75 | 0.56 | 1.99 | 3.55 | 1.78 | 42.07 |