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Table 1 List of the oligonucleotides used in this study

From: Environmental detection of eumycetoma pathogens using multiplex real-time PCR for soil DNA in Sennar State, Sudan

Target species

Primer/probe

Sequencea

Product size

Madurella mycetomatis

M.m_forward

5′-CCTCCCGGTAGTGTAGTGTCC-3′

89 bp

M.m_reverse

5′-GAGAGGCCGTACAGAGCAAAT-3′

 

M.m_probe

5′-FAM-GGCGTCCGCCGGAGGATTATACAAC-BHQ1-3′

 

Falciformispora senegalensis

F.s_forward

5′-GTTCCTACGCCGGCAAC-3′

119 bp

F.s_reverse

5′-AGACAGGTATACTGCTTTTGCTGC-3′

 

F.s_probe

5′-HEX- GCCGCTGGGTCTCCACC-BHQ1-3′

 

Falciformispora tompkinsii

F.t_forward

5′-CTTTGGCTCTGCCACTGC-3′

123 bp

F.t_reverse

5′-GACAAGTGTACTGCTTCTAACGGC-3′

 

F.t_probe

5′-TAMRA- TCTGCCGCTGGGCATCTTAAT -BHQ2-3′

 
  1. aFAM: fluorescein amidite, BHQ: Black Hole Quencher, HEX: hexachloro-fluorescein, TAMRA: carboxytetramethyl rhodamine