Fig. 1

Map of sampling location with the result of multiplex PCR. Each point on the map presents a sample collection site. Letters refer to the sampling villages: A Deshein, B Sharfa Al-Mihrab, C Al-Awia, D Wad Hassan, E Wad Ajeeb, F Al-Ragal Al-Batahin and G Wad Al-har. Pie charts indicate the detection of the target species: red color: Madurella mycetomatis; blue color: Falciformispora senegalensis; and green color: Falciformispora tompkinsii. Map data were downloaded from the OpenStreetMap project (OpenStreetMap contributors) under a CC BY-SA 2.0 license (www.openstreetmap.org). The map was created using the QGIS Geographic Information System, Open Source Geospatial Foundation Project, under a CC BY-SA 3.0 license (http://qgis.osgeo.org)