Fig. 10

Elevated intracellular levels of reactive oxygen species in Trypanosoma cruzi-infected cardiomyocytes. A Signal intensity of reactive oxygen species-specific fluorescence was higher in the cardiomyocytes infected with the Y strain at 24-h post-infection, compared with non-infected control cells. Statistical comparison was performed with Mann–Whitney test. Bars represent mean fluorescence intensity and error bars represent standard deviation. B Representative fluorescence microscopy images from reactive oxygen species-specific CellRox green staining. Comparison with the non-infected (upper) control cells shows higher intensity of reactive oxygen species fluorescence signal in the cardiomyocytes 24-h post-infection with Trypanosoma cruzi Y strain (lower). Scale bars represent 100 µm