Fig. 3

CD4⁺ and CD8⁺ T cells population in immunized mice. a Experimental procedure for cellular immune response. C57BL/6 mice (n = 5) were immunized with Z_EDIII or PBS at 0, 14, and 42 dpi. Mice were euthanized at 7, 17, and 45 dpi (7 dpi; b–d, 17 dpi; e–g, 45 dpi; h–j). Total splenocytes were collected from five mice in each group and stained with antibodies to CD3, CD4, and CD8 molecule