Fig. 4

Number of IFN-γ-secreting splenocytes in immunized mice. C57BL/6 mice (n = 5) were immunized with Z_EDIII or PBS at 0, 14, and 42 dpi. Mice were euthanized 7, 17, and 45 dpi (7 dpi; a, 17 dpi; b, 45 dpi; c). Splenocytes were stimulated with Z_EDIII (10 μg/ml) or PBS. Ag-specific IFN-γ response was identified by ELISPOT assay at 48 h after stimulation. Data show mean ± SD of five mice per group. Each sample was assayed in triplicate. Splenocytes were pooled from five mice in each group. Statistical analysis *p < 0.05, **p < 0.01, ***p < 0.001