Fig. 3

RT-LAMP-based detection of ZIKV from a Zika fever patient. a Scheme of preparing patient samples for RT-LAMP. RNA extracted from each patient sample (urine, serum (acute phase), and serum (remission phase)) was provided for ZIKV detection in the RT-LAMP reaction for 60 min at 62 °C. Water served as a negative control in the RT-LAMP reaction. RNA from ZIKV MR and PR served as positive controls. b Amplified products were electrophoresed on a 2% agarose gel. Numbers on the left indicate migration of the molecular weight marker (bp). c Amplification of the target sequence contained in patient samples was monitored using a real-time turbidimeter (turbidity at 650 nm)