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Fig. 4 | Tropical Medicine and Health

Fig. 4

From: Identification of novel antiviral of fungus-derived brefeldin A against dengue viruses

Fig. 4

Time-of-addition studies. a Schematic illustration of time-of-addition studies for treatment with BFA. Vero cells in 96-well plates were treated with 125 nM BFA at different time points before, during, and after DENV infection (MOI of 5). For after-infection assay, BFA was added at seven different time points after virus exposure. b After 24 h postinfection, released virus titer was determined by focus assay as described in the “Materials and methods” section. Error bars represent standard errors of two independent experiments

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