Fig. 4
From: Identification of novel antiviral of fungus-derived brefeldin A against dengue viruses
Time-of-addition studies. a Schematic illustration of time-of-addition studies for treatment with BFA. Vero cells in 96-well plates were treated with 125 nM BFA at different time points before, during, and after DENV infection (MOI of 5). For after-infection assay, BFA was added at seven different time points after virus exposure. b After 24 h postinfection, released virus titer was determined by focus assay as described in the “Materials and methods” section. Error bars represent standard errors of two independent experiments