Development of a simple and specific direct competitive ELISA for the determination of artesunate using an anti-artesunate polyclonal antiserum

Table 3 Recovery of artesunate (ART) added to tablet sample solutions estimated by the ELISA

Sample	Added ART (ng/ml)	Estimated ART^a (ng/ml)	Coefficient of variation^b (%)	Recovery^c (%)
Intra-assay (n = 6)	0	7.6 ± 0.4	5.2	–
	10	17.5 ± 0.8	4.8	99.0
	50	56.8 ± 2.3	4.1	98.3
Inter-assay (n = 12)	0	7.6 ± 0.4	5.1	–
	10	18.2 ± 1.3	7.0	105.7
	50	57.8 ± 2.2	3.8	100.3

n number of determinations
^aMean ± standard deviation, a concentration of 10 ng/ml is equivalent to 50 mg of a tablet sample
^bCoefficient of variation = 100 × standard deviation/mean
^cRecovery (%) = 100 × (mean ART amounts estimated − mean estimated ART amounts in a tablet sample solution alone)/ART amounts added

ISSN: 1349-4147