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Table 3 Recovery of artesunate (ART) added to tablet sample solutions estimated by the ELISA

From: Development of a simple and specific direct competitive ELISA for the determination of artesunate using an anti-artesunate polyclonal antiserum

Sample Added ART (ng/ml) Estimated ARTa (ng/ml) Coefficient of variationb (%) Recoveryc (%)
Intra-assay (n = 6) 0 7.6 ± 0.4 5.2
10 17.5 ± 0.8 4.8 99.0
50 56.8 ± 2.3 4.1 98.3
Inter-assay (n = 12) 0 7.6 ± 0.4 5.1
10 18.2 ± 1.3 7.0 105.7
50 57.8 ± 2.2 3.8 100.3
  1. n number of determinations
  2. aMean ± standard deviation, a concentration of 10 ng/ml is equivalent to 50 mg of a tablet sample
  3. bCoefficient of variation = 100 × standard deviation/mean
  4. cRecovery (%) = 100 × (mean ART amounts estimated − mean estimated ART amounts in a tablet sample solution alone)/ART amounts added