Table 1 Components of mixture for rabies PCR1100 assay

RNase-free water

0.27

µL

2 × KAPA3G Plant Buffer

8.5

µL

LN34 Forward

709 nM

1.2

µL

LN34 Reverse

709 nM

1.2

µL

LN34 Probe

200 nM

0.68

µL

MgCl₂ (KAPA3GPlant)

1.15

µL

DNA Polymerase (KAPA 3GPlant)

1

µL

FastGene™ Scriptase II

1

µL

RNA template

2

µL

17

µL

RNase-free water

0.27

µL

2 × KAPA3G Plant Buffer

8.5

µL

LN34 Forward

497 nM

0.84

µL

LN34 Reverse

497 nM

0.84

µL

LN34 Probe

140 nM

0.476

µL

β-actin Forward

212 nM

0.36

µL

β-actin Reverse

212 nM

0.36

µL

β-actin Probe

60 nM

0.204

µL

MgCl₂ (KAPA3GPlant)

1.15

µL

DNA Polymerase (KAPA 3GPlant)

1

µL

FastGene™ Scriptase II

1

µL

RNA template

2

µL

17

µL