Fig. 2

ZIKV envelope protein domain III-specific antibody responses elicited by Z_EDIII. a Experimental strategies. C57BL/6 mice (n = 5 per group) were immunized with Z_EDIII or PBS at 0, 14, and 42 dpi. For Ag-specific IgG antibodies, blood samples were collected at 0, 28, and 49 dpi. b Serum samples were diluted (1:50,000) and OD 450 values were measured using ZIKV envelope protein domain III-coated ELISA kit. The ZIKV envelope protein domain III-specific IgG antibody activity units were calculated. c Serum samples were diluted (1:100) and OD 450 values were measured. d IgG2c-to-IgG1 ratio in Z_EDIII- or PBS-immunized mice is shown. Data show the mean ± SD of five mice per group. Each sample was assayed in duplicate. Statistical analysis *p < 0.05, **p < 0.01, ***p < 0.001