Skip to main content
Fig. 3 | Tropical Medicine and Health

Fig. 3

From: A simple and affordable method for estimating the fluid volume a mosquito sucks using food dyes

Fig. 3

Colorimetric quantification of ATP solution ingested by mosquitoes. a Absorbance at 638 nm of two-fold serial dilutions of green dye which was dissolved in ultrapure water. Two hundred microliter of each solution containing the indicated concentration of green dye was applied to a well of 96-well plate. Simple linear regression analysis was performed (R2 = 0.9936). b Absorbance at 638 nm of homogenized solution prepared from each Ae. aegypti female mosquito that sucked 0.1 mM ATP solution containing either 0 μg/μl, 4 μg/μl, or 20 μg/μl of green dye. All experiments were performed more than three times. The numbers of mosquitoes used at each concentration of dye were 89, 83, and 74, respectively. All error bars show standard error of mean (SEM), and two bars in this figure were not visible due to the small values of SEM. Simple linear regression analysis was performed (R2 = 0.8885). The average absorbance of mosquitoes fed with ATP solution (20 μg/μl green dye) was 0.3539. c Absorbance at 638 nm of PBS containing green dye. Each aliquot (0.5–8 μl) of 20 μg/μl green dye was added to a 1.5-ml tube containing 230 μl of PBS, and then 200 μl of each solution was transferred to a 96-well plate. All error bars show SEM. Simple linear regression derived a formula to estimate how much volume of ATP solution a mosquito sucks

Back to article page