Fig. 5

NMDS ordination (k = 3, stress = 0.141) of Bray-Curtis pairwise distances of bacterial communities from drinking water sources collected in the study (either home or school locations). Each water source was sampled with a kit consisting of three tandem filters (Filter A and Filter B), yielding three biological replicates of a water source per kit. Each point represents backflushed contents from Filter A, Filter B, laboratory backflush controls, mock community controls, or negative controls. Each kit is represented by up to 3 Filter A points and 3 Filter B points. Samples with fewer than 5000 sequencing reads were not included in the analysis. The same ordination is used for all three panels. a All backflush samples included in the study and associated controls. Shape and color delineate whether a sample is from Filter A, Filter B, or a control. b A subset of four kits selected to show the relationship between biological replicates of Filter A and Filter B within and between kits. Shape delineates Filter A, Filter B, and control samples. Color delineates which kit (i.e. water source) each filter sample represents along with the type of control. c The same kits as shown in b with identical coloring showing kit membership. Shape delineates the type of water source