Fig. 4
Sensitivity of RT-LAMP for the detection of ZIKV from Zika fever patient. a Scheme for preparing the ZIKV PATIENT strain from patient’s urine for RT-LAMP detection. b, c Amplification of ZIKV NS3 from the MR and PATIENT strains by RT-LAMP. RNA of each ZIKV strain (3 × 102, 3 × 101, 3 × 100, 3 × 10−1, 3 × 10−2, 3 × 10−3, and 3 × 10−4 ng) was used as a template for RT-LAMP reactions for 60 min at 62 °C. Water served as a negative control in the RT-LAMP reaction. b Agarose gel electrophoresis of the RT-LAMP amplified products. The reaction mixtures were electrophoresed on a 2% agarose gel. Numbers of the left indicate migration of the molecular weight marker (bp). c Amplification of the target sequence of the MR and PATIENT strains was monitored using a real-time turbidimeter (turbidity at 650 nm)