Fig. 2
Sensitivity of RT-LAMP for the detection of ZIKV MR and PR strains. RNA from each ZIKV strain (3 × 102, 3 × 101, 3 × 100, 3 × 10−1, 3 × 10−2, 3 × 10−3, and 3 × 10−4 ng) was used as a template for RT-LAMP reactions for 60 min at 62 °C. Water served as a negative control in RT-LAMP reaction. a Agarose gel electrophoresis of the RT-LAMP amplified products. The reaction mixtures were electrophoresed on a 2% agarose gel. Numbers on the left indicate migration of the molecular weight marker (bp). b Amplification of ZIKV NS3 of the MR and PR strains monitored using a real-time turbidimeter (turbidity at 650 nm)