Table 1 Oligonucleotide sequences used in this study
Gene | Target | Sequence of primers (5′ to 3′) | Melting (Tm) | Product size (bp) | Ref |
|---|---|---|---|---|---|
divIVA | E. faecalis | F: ACGTGTCTTCCATCAACGCT R: ACTGCTGTATGTTTGTCTCCGA | 79.9 | 123 | [11] |
Alanine racemase | E. faecium | F: ATCCCTCTGGGCACGCAC R: ACATACACGCCCAATCGTTTC | 85.4 | 248 | [11] |
vanA | Vancomycin | F: GCTGTGAGGTCGGTTGTG R: GCTCGACTTCCTGATGAATACG | 82.9 | 101 | [11] |