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Fig. 6 | Tropical Medicine and Health

Fig. 6

From: Development of high-resolution melting curve analysis in rapid detection of vanA gene, Enterococcus faecalis, and Enterococcus faecium from clinical isolates

Fig. 6

HRMA graphs corresponding to one high-resolution melting analysis of a subset of clinical specimens (n = 18). Curves of tested samples previously identified as E. faecalis are shown in black lanes. DNA samples in this study were prepared and amplified successfully using the EvaGreen dye-based method in the ABI instrument. Primer-specific melting peaks (Tm) were obtained via HRM analysis, allowing the differentiation of all investigated β-lactamase enzymes. Due to the highly saturating EvaGreen dye and the HRMA analysis, the accuracy of the resolution was ± 0. 1–0.5 °C

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Tropical Medicine and Health

ISSN: 1349-4147

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