Fig. 2
Sensitivity of real-time PCR for primers used to detect of E. faecium (alanine racemase gene) with a melting point of 85.4 ± 0.5 °C and amplification curve. E. faecium NCTC7174 (as standard bacteria) with the concentration of 0.5 McFarland (1.5 × 108 CFU/ml) was provided into serial dilutions of 107 to 100 CFU/ml. a 107, b 106, c 105, d 104, e 103, f 102, g 101, and h 100. a Amplification curve b Melting curve profile, and c corresponding agarose gel (1.5%) electrophoresis for real-time PCR amplification of alanine racemase gene. Horizontal lines represent cycle threshold of real-time PCR. One peak with a shoulder corresponds to genomic DNA amplification; no peak corresponds to no amplification. SYBR Green I color and single-tube reaction were used in this test. Also, real-time PCR was performed as single step