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Table 2 Primer and probe sequences used to detect virulence factor RNA transcripts by real-time PCR

From: Virulence factor RNA transcript expression in the Leishmania Viannia subgenus: influence of species, isolate source, and Leishmania RNA virus-1

Target Sequence
18S
 Forward 5′-AAGTGCTTTCCCATCGCAACT-3′
 Reverse 5′-GACGCACTAAACCCCTCCAA-3′
 Probe FAM-CGGTTCGGTGTGTGGCGCC-NFQ
GP63
 Forward 5′-GGCTTCTACCAGGCGGACTT-3′
 Reverse 5′-TGATGYbYbBTBCRaCCATGCACTT-3′
 Probe FAM-AGGCCGAGGTGATG-MBG
CPB
 Forward 5′-GCTCGTCGGGTACAACAAGAC-3′
 Reverse 5′-AGTCCTCACCCCACGAGTTCT-3′
 Probe FAM-TTCCGTACTGGGTGATC-BHQ1
MPI
 Forward 5′-GCTGCGAGGCCGGATAA-3′
 Reverse 5′-GGAGTCAAGGCGCARaATGAG-3′
 Probe FAM-TACAAGGACCCGAACCACARaGCCTGA-BHQ1
HSP23
 Forward 5′-GARaCGScTGCTTCGAGCTT-3′
 Reverse 5′-GAAGScTGGCCTTGATTTTGC-3′
 Probe FAM-CTGTTCGAGCTTC-BNFQ
HSP70
 Forward 5′-GTGGAWdATCATCGCGAACGA-3′
 Reverse 5′-GAGTCCGTGAACGCAACGTA-3′
 Probe FAM-AGGGYbAACCGCACGACACCGT-BHQ1
HSP90
 Forward 5′-CAAGAAGCGCAACAACATCAA-3′
 Reverse 5′-TCGCAGTTGTCCATGATGAAC-3′
 Probe FAM-TGTACGTGCGCCGCG-BHQ1
HSP100
 Forward 5′-CCGACTTCCARaGACGACAAC-3′a
 Reverse 5′-GCCTGCTTGCAGAGATCRaA-3′
 Probe FAM-ACGAGTCACTGAACAAG-BHQ1
  1. aR = A,G
  2. bY = C,T
  3. cS = C,G
  4. dW = A,T