Table 2 Primer and probe sequences used to detect virulence factor RNA transcripts by real-time PCR

 Forward

5′-AAGTGCTTTCCCATCGCAACT-3′

 Reverse

5′-GACGCACTAAACCCCTCCAA-3′

 Probe

FAM-CGGTTCGGTGTGTGGCGCC-NFQ

 Forward

5′-GGCTTCTACCAGGCGGACTT-3′

 Reverse

5′-TGATGY^bY^bBTBCR^aCCATGCACTT-3′

 Probe

FAM-AGGCCGAGGTGATG-MBG

 Forward

5′-GCTCGTCGGGTACAACAAGAC-3′

 Reverse

5′-AGTCCTCACCCCACGAGTTCT-3′

 Probe

FAM-TTCCGTACTGGGTGATC-BHQ1

 Forward

5′-GCTGCGAGGCCGGATAA-3′

 Reverse

5′-GGAGTCAAGGCGCAR^aATGAG-3′

 Probe

FAM-TACAAGGACCCGAACCACAR^aGCCTGA-BHQ1

 Forward

5′-GAR^aCGS^cTGCTTCGAGCTT-3′

 Reverse

5′-GAAGS^cTGGCCTTGATTTTGC-3′

 Probe

FAM-CTGTTCGAGCTTC-BNFQ

 Forward

5′-GTGGAW^dATCATCGCGAACGA-3′

 Reverse

5′-GAGTCCGTGAACGCAACGTA-3′

 Probe

FAM-AGGGY^bAACCGCACGACACCGT-BHQ1

 Forward

5′-CAAGAAGCGCAACAACATCAA-3′

 Reverse

5′-TCGCAGTTGTCCATGATGAAC-3′

 Probe

FAM-TGTACGTGCGCCGCG-BHQ1

 Forward

5′-CCGACTTCCAR^aGACGACAAC-3′^a

 Reverse

5′-GCCTGCTTGCAGAGATCR^aA-3′

 Probe

FAM-ACGAGTCACTGAACAAG-BHQ1