Comparison of virulence gene profiles and genomic fingerprints of Vibrio cholerae O1 and non-O1/non-O139 isolates from diarrheal patients in southern Thailand

Table 2 PCR primers used in this study

Target genes	Protein product	Sequence (5′–3′)	Product size (bp)^a	Reference
ctxA	Cholera toxin	CGGGCAGATTCTAGACCTCCTG CGATGATCTTGGAGCATTCCCAC	564	[45, 46]
tcpA	Toxin-coregulated pili	CACGATAAGAAAACCGGTCAAGAG CGAAAGCACCTTCTTTCACACGTTG TTACCAAATGCAACGCCGAATG	453 (ET) 620 (C)	[28, 46]
zot	Zonula occludens toxin	TCGCTTAACGATGGCGCGTTTT AACCCCGTTTCACTTCTACCCA	947	[28, 46]
ace	Accessory cholera enterotoxin	TAAGGATGTGCTTATGATGGACACCC CGTGATGAATAAAGATACTCATAGG	316	[47]
stn/sto	Heat-stable enterotoxin	TCGCATTTAGCCAAACAGTAGAAA GCTGGATTGCAACATATTTCGC	172	[28]
hlyA	El Tor Hemolysin	GGCAAACAGCGAAACAAATACC GAGCCGGCATTCATCTGAAT CTCAGCGGGCTAATACGGTTTA	481 (ET) 738/727 (ET/C)	[28]
vcsV2	ATPase (T3SS)	ATGCAGATCTTTTGGCTCACTTGATGGG ATGCGTCGACGCCACATCATTGCTTGCT	742	[6]
vasH	Transcriptional regulator (T6SS)	TGTTGATGGGCGAGAGTCAC ACGTGTGTGGCAGATACCAG	631	[31]

ISSN: 1349-4147