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Fig. 2 | Tropical Medicine and Health

Fig. 2

From: Identification of novel antiviral of fungus-derived brefeldin A against dengue viruses

Fig. 2

Identification of anti-DENV activity of brefeldin A by virus titration and immunofluorescence assay or IFA. a Vero cells in 96-well plates were infected with DENV at an MOI of 0.5 in the presence of compound at indicated concentrations or 0.1% DMSO. After 24 h or 48 h incubation, ICF was harvested and virus titer was determined by focus assay as described in the “Materials and methods” section. In separated experiments, Vero cells in 96-well plates were treated with the compounds at different concentrations without virus infection. After 24 h or 48 h incubation, cell viability was determined by MTT assay as described in the “Materials and methods” section. b Vero cells in 24-well plates were infected with DENV at an MOI of 0.5 and added with the compounds at indicated concentrations. After 48 h incubation, the cells were recovered, spotted onto glass slides, and stained with 12D11 monoclonal antibody which react to viral envelope protein

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