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Table 2 Precision and accuracy of a direct competitive ELISA of artesunate (ART)

From: Development of a simple and specific direct competitive ELISA for the determination of artesunate using an anti-artesunate polyclonal antiserum

Sample Added ART (ng/ml) Estimated ARTa (ng/ml) Coefficient of variationb (%) Recoveryc (%)
Intra-assay (n = 5) 0.1 0.10 ± 0.03 25.5 106.0
0.3 0.30 ± 0.01 3.9 99.0
1.0 1.00 ± 0.02 2.3 99.6
3.0 2.9 ± 0.1 2.9 97.3
10.0 10.1 ± 0.2 2.4 100.8
30.0 29.3 ± 1.7 5.9 97.7
Inter-assay (n = 10) 0.1 0.10 ± 0.02 22.0 103.0
0.3 0.30 ± 0.01 4.3 99.3
1.0 1.00 ± 0.02 2.4 99.9
3.0 3.0 ± 0.1 2.7 98.7
10.0 10.1 ± 0.3 3.2 100.9
30.0 29.2 ± 1.8 6.3 97.2
  1. n number of determinations
  2. aMean ± standard deviation
  3. bCoefficient of variation = 100 × standard deviation/mean
  4. cRecovery (%) = 100 × (mean estimated amounts/added amounts)