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Table 2 Precision and accuracy of a direct competitive ELISA of artesunate (ART)

From: Development of a simple and specific direct competitive ELISA for the determination of artesunate using an anti-artesunate polyclonal antiserum

Sample

Added ART (ng/ml)

Estimated ARTa (ng/ml)

Coefficient of variationb (%)

Recoveryc (%)

Intra-assay (n = 5)

0.1

0.10 ± 0.03

25.5

106.0

0.3

0.30 ± 0.01

3.9

99.0

1.0

1.00 ± 0.02

2.3

99.6

3.0

2.9 ± 0.1

2.9

97.3

10.0

10.1 ± 0.2

2.4

100.8

30.0

29.3 ± 1.7

5.9

97.7

Inter-assay (n = 10)

0.1

0.10 ± 0.02

22.0

103.0

0.3

0.30 ± 0.01

4.3

99.3

1.0

1.00 ± 0.02

2.4

99.9

3.0

3.0 ± 0.1

2.7

98.7

10.0

10.1 ± 0.3

3.2

100.9

30.0

29.2 ± 1.8

6.3

97.2

  1. n number of determinations
  2. aMean ± standard deviation
  3. bCoefficient of variation = 100 × standard deviation/mean
  4. cRecovery (%) = 100 × (mean estimated amounts/added amounts)