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Table 2 Primers and probes for real-time PCR

From: Detection of Mycobacterium ulcerans by real-time PCR with improved primers

Primer or probe Sequence (5′–3′)
IS2404TF AAAGCACCACGCAGCATCT
IS2404TR AGCGACCCCAGTGGATTG
IS2404TP FAM-CGTCCAACGCGATC-BHQ1
IS2404KF TCTCGTGTCGGTGTTC
IS2404KR TGACGACCTGGGTATG
IS2404KP FAM-AATGAAATTCCCTGCGT-MGB
  1. IS2404TF, IS2404TR, and IS2404TP were described by Fyfe et al. [18]
  2. FAM fluorescein amidite, BHQ1 black hole quencher 1, MGB minor groove binder