Fig. 3
From: Detection of Mycobacterium ulcerans by real-time PCR with improved primers
Specificity of the new IS2404 primer/probe set for M. ulcerans detection. Real-time PCR was performed with the conventional primers and probe (IS2404TF, IS2404TR, and IS2404TP) or the new primers and probe (IS2404KF, IS2404KR, and IS2404KP) using 10 ng of genomic DNA of each M. ulcerans strain as a template. The results are expressed as the mean ± standard deviation of the threshold cycle based on three independent experiments; **p < 0.01 by Student’s t test, nd: not detected (threshold cycle over 33)