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Fig. 2 | Tropical Medicine and Health

Fig. 2

From: Development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against dengue 2 viral protease

Fig. 2

Substrate saturation assay. Fluorogenic substrate was serially diluted prior to addition of 1 μg/mL purified recombinant DENV2 NS2B3pro. Baseline values at time 0 were subtracted. Two nonlinear regression models of enzyme kinetics were plotted; Michaelis-Menten model (black dashed line) and substrate inhibition model (red line). Inset shows Lineweaver-Burk plot of the same. Error bars indicate standard deviation (SD)

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