Table 2 Primers for the determination of SCCmec type of MRSA isolates
Primer | Nucleotide sequence (5′ to 3′) | Product size (bp) | SCCmec type/region |
|---|---|---|---|
CIF2 F2 | TTCGAGTTGCTGATGAAGAAGG | 495 | I, IA |
CIF2 R2 | ATTTACCACAATTACTACCAGC | ||
KDP F1 | AATCATCTGCCATTGGTGATGC | 284 | II |
KDP R1 | CGAATGAAGTGAAAGAAAGTGG | ||
DCS F2 | CATCCTATGATAGCTTGGTC | 342 | I, IA, II, IV, IVA |
DCS R2 | CTAAATCATAGCCATGACCG | ||
RIF4 F3 | GTGATTGTTCGAGATATGTGG | 243 | III |
RIF4 R9 | CGCTTTATCTGTATCTATCGC | ||
IS431 P4 | CAGGTCTCTTCAGATCTACG | 381 | IA, II, IVA |
PUB110 R1 | GAGCCATAAACACCAATAGCC | ||
MECA P4 | TCCAGATTACAACTTCACCAGG | 162 | mecA gene |
MECA P7 | CCACTTCATATCTTGTAACG |