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Table 1 Michaelis constant (K m ) approximation

From: Development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against dengue 2 viral protease

 

Michaelis-Menten model

Substrate inhibition model

Max substrate conc.

1000 μM

500 μM

1000 μM

500 μM

K m in μM (95 % CI)

51.23

(36.80 to 65.66)

76.24

(58.24 to 94.24)

135.0

(99.52 to 170.4)

171.7

(88.73 to 254.6)

Adjusted R square

0.8965

0.9638

0.9687

0.9796

  1. Estimates of K m derived from varied nonlinear regression models and substrate maximal concentrations at initial velocity conditions were presented