Fig. 2

Substrate saturation assay. Fluorogenic substrate was serially diluted prior to addition of 1 μg/mL purified recombinant DENV2 NS2B3pro. Baseline values at time 0 were subtracted. Two nonlinear regression models of enzyme kinetics were plotted; Michaelis-Menten model (black dashed line) and substrate inhibition model (red line). Inset shows Lineweaver-Burk plot of the same. Error bars indicate standard deviation (SD)